Table 2. Primer ID and GenBank Accession numbers, author affiliation, allele ranges and PCR reaction forward and reverse primer quantities for 11 microsatellite markers used during the genotyping of P. crucifer from 2008 to 2011.

Primer ID	Forward sequence (5′ to 3′)	Reverse sequence (3′ to 5′)	Ta	GenBank accession no.	Reference	Allele range	Repeat motif	Quantity PCR reaction primer
Pcru32	CCCTACATAGGATTG	GTACACCCACCAAAAGTGCT	54	EF190904	Van Buskirk et al. (2006, unpublished)	121–163	(CA)11	0.2 μl
Pcru10	GGGGGATGCAGAATT	CGGTTCCTATTGAAGAACA	54	EF190896	Degner et al. (2009)	187–213	(CA)13	0.5 μl
Pcru05	CATTTATAAGCAGTGCAGAGAGG	TGCATTGATGTTTCTCATGG	54	EF190892	Van Buskirk et al. (2006, unpublished)	320–366	(CA)13	0.5 μl
Pcru11	GGATATGCTCACATG	CCCAGATTCCAAGTGTTTTC	55	EF190897	Van Buskirk et al. (2006, unpublished)	112–168	(GT)16	0.3 μl
Pcru21	TGGAGACATCATTGC	CCCTTGGTCCTGAATAGGTT	55	EF190900	Van Buskirk et al. (2006, unpublished)	210–264	(CA)14	0.45 μl
Pcru09	GGGGGATGCAGAATT	CGGTTCCTATTGAAGAACA	54	EF190896	Degner et al. (2009)	125–159	(CA)15	0.2 μl
Pcru14	GATCAGACAGTCTACAGTAATGAGGAG	CATAACACAGGGCAACCAAG	54	EF190899	Degner et al. (2009)	184–232	(GT)13	0.3 μl
Pcru06	CATTTACAAACGGCACTGCTC	CCCCAGTCATCAGGAATACA	54	EF190893	Van Buskirk et al. (2006, unpublished)	186–236	(GT)16	0.3 μl
Pcru12	TCAAATTGACCATCCATCC	GCCAGCCCCTATAGGATTAG	54	EF 190898	Van Buskirk et al. (2006, unpublished)	114–166	(GT)13	0.5 μl
Pcru24	TGCCATGGGGATGTTATATG	CGAGCTATAGGAAAAGGCAGAG	54	EF190902	Degner et al. (2009)	96–146	(CA)17	0.35 μl
Pcru08	CTAACCGTAGCAGGGAGGTTG	TAGGACTGAGGGAGGAGAGG	54	EF 190894	Van Buskirk et al. (2006, unpublished)	222–270	(CA)12	0.5 μl