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. 2016 Mar 24;11(3):e0152206. doi: 10.1371/journal.pone.0152206

Fig 2. Pkp1 knockdown inhibits tooth germ growth and dental epithelial cell proliferation.

Fig 2

A, CLDE cell proliferation 3 days after treatment with control or Pkp1 siRNA, as determined by CCK-8 assay. B, BrdU incorporation by CLDE cells with or without Pkp1 knockdown. The ratio was calculated as BrdU-positive cell number/DAPI-stained nuclei. C, Seven-day organ cultures of E13 tooth germs transfected with control or Pkp1 siRNA. D, Relative tooth size plot (n = 12), with the average tooth germ size in the control siRNA group set at 1.0. E, qRT-PCR analysis of Pkp1 expression in cultured tooth germ after normalization to Gapdh mRNA expression. F, CLDE cell proliferation in the presence of Wnt3a was evaluated using a CCK-8 assay after transfection with control or Pkp1 siRNA. G, TCF/LEF promoter activity after stimulation with Wnt3a was examined using a luciferase assay after transfection with control or Pkp1 siRNA. *P <0.01, Error bars represent the mean ± S.D.