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. 2016 Mar 24;11(3):e0151848. doi: 10.1371/journal.pone.0151848

Fig 3. LEC ITGA2b is not required for residence in fibronectin-rich environments.

Fig 3

(A) Flow cytometry histograms show the percentage ±SD (n = 4) of LECs expressing ITGB3 by the four stromal subsets. (B) Confocal microscopic images of a LN subcapsular area labelled for fibronectin (magenta) and ITGA2b (green). Nuclear coloration was with DAPI. (C) Confocal high resolution microscopy of the subcapsular area labelled for ITGA2b and mCLCA1 (mAb 10.1.1). (D) Histograms show recognition of the active conformation of the ITGA2b/ITGB3 complex on activated platelets but not on LECs or unstimulated platelets by the PE-conjugated JON/A mAb. (E) Histograms show ITGAV expression ±SD (n = 3) on the stromal subsets. (F) Flow cytometry counterplots of LECs probed for expression of ITGA2b and ITGB3 in Itga2b+/+, Itga2b+/- and Itga2b-/- mice (n = 3).