Fig 7. Comparison of amino acid sequences of BGL1, BGL2, and BGL3 from Clavispora NRRL Y-50464 with β-glucosidases from other species of Aspergillus niger (AnBGL1), Trichoderma harzianum (ThBGL2), Trichoderma reesei (TrBGL), Kluyveromyces marxianus (KmBGL1), Myceliophthora thermophila (MtBGL3a), and Bacillus polymyxa (BpBGLb).

Matched consensus sequences are shaded in black with a white font. Strongly conserved sequence and weak conserved sequence is marked with a solid and open triangle, respectively. A conserved motif of FVMSDW for glycosyl hydrolase family 3 is indicated by a bold line. Conserved acidic residue found in A. niger is marked with a star. Important residues of catalytic nucleophile located at Asp225, Asp224, and Asp225 the N-terminal domain and the proton donor Glu449, Glu459, and Glu459 in the C-terminal domain were boxed respectively for the three BGLs from strain Y-50464.