Table 2. Kinetic parameters of a partially purified protein of BGL2 or BGL3 from Clavispora NRRL Y-50464 using pNPG as a substrate.
Protein | Vmax (μmol min-1 mg-1) | Kcat (min-1) | Kcat/Km (mM-1 min-1) | Km (mM) | K i (mM) (against glucose) | Reference |
---|---|---|---|---|---|---|
BGL2 | 5.27 ± 0.11 | 547.89 ± 15.91 | 6,834.23 ± 94.47 | 0.08 ± 0.01 | 61.97±2.49 | This work |
BGL3 | 1.63 ± 0.04 | 84.04 ± 1.06 | 462.50 ± 14.12 | 0.18 ± 0.02 | 38.33±1.15 | This work |
BGL1 | 5.91 | na* | na | 0.355 | 15.2 | [11] |
Novo188 | 4.2 | na | na | 0.448 | 0.735 | [11] |
*na: not available. Data from this work are presented by mean values with standard deviations (n = 3).