Table 3. Specific activity of partially purified BGL2 and BGL3 from Clavispora NRRL Y-50464 towards selective substrate of oligosaccharides.
Oligosaccharides | Linkage of glycosyl group | BGL2 | BGL3 | ||
---|---|---|---|---|---|
Specific activity (U/mg) | Relative activity (%) | Specific activity (U/mg) | Relative activity (%) | ||
Cellobiose | β (1,4)-Glucose | 1.74 ± 0.06 | 100 | 0.66 ± 0.02 | 100 |
Cellotriose | β (1,4)-Glucose | 1.35 ± 0.04 | 77.59 | 0.80 ± 0.02 | 121.21 |
Cellotetraose | β (1,4)-Glucose | 1.14 ± 0.03 | 65.52 | 0.62 ± 0.01 | 93.94 |
Cellopentaose | β (1,4)-Glucose | 1.53 ± 0.04 | 87.93 | 0.81 ± 0.02 | 122.73 |
Laminaribiose | β (1,3)-Glucose | 1.56 ± 0.06 | 89.66 | 0.82 ± 0.01 | 124.24 |
Laminaritriose | β (1,3)-Glucose | 1.28 ± 0.04 | 73.56 | 0.78 ± 0.02 | 118.18 |
Laminaritetraose | β (1,3)-Glucose | 1.33 ± 0.04 | 76.44 | 0.76 ± 0.02 | 115.15 |
Laminaripentaose | β (1,3)-Glucose | 1.33 ± 0.04 | 76.44 | 0.76 ± 0.02 | 115.15 |
Laminarin | β (1,3)-Glucose | 0.91 ± 0.03 | 52.30 | 0.52 ± 0.01 | 78.79 |
Lactose | β 1-galactose, α 4-glucose | 1.14 ± 0.04 | 65.52 | 0.71 ± 0.01 | 107.58 |
Lichenan | β (1,3–1,4)-Glucose | 1.40 ± 0.06 | 80.46 | 0.73 ± 0.01 | 110.61 |
Gentiobiose | β (1,6)-Glucose | 1.03 ± 0.03 | 59.20 | 0.73 ± 0.01 | 110.61 |
Salicin | β-Glucose | 1.17 ± 0.03 | 67.24 | 0.69 ± 0.01 | 104.55 |
Sophorose | β (1,2)-Glucose | 1.26 ± 0.03 | 72.41 | 0.75 ± 0.01 | 113.64 |