Table 4. Effects of metal ions and chemicals on specific activity of partially purified BGL2 and BGL3 from Clavispora NRRL Y-50464 using pNPG as a substrate.
| Treatment | BGL2 | BGL3 | ||
|---|---|---|---|---|
| Specific activity (U/mg) | Relative activity (%) | Specific activity (U/mg) | Relative activity (%) | |
| Control | 3.95 ± 0.07 | 100 | 1.96 ± 0.02 | 100 |
| NaCl (1 mM) | 6.86 ± 0.12 | 173.67 | 2.01 ± 0.03 | 102.55 |
| KCl (1 mM) | 6.76 ± 0.13 | 171.14 | 2.08 ± 0.03 | 106.12 |
| CaCl2 (1 mM) | 3.86 ± 0.09 | 97.72 | 2.13 ± 0.02 | 108.67 |
| ZnCl2 (1 mM) | 4.12 ± 0.10 | 104.3 | 2.68 ± 0.03 | 136.73 |
| MgCl2 (1 mM) | 4 ± 0.08 | 101.26 | 2.05 ± 0.02 | 104.59 |
| CuCl2 (1 mM) | 2.96 ± 0.03 | 74.94 | 1.67 ± 0.01 | 85.2 |
| CoCl2 (1 mM) | 4.15 ± 0.11 | 105.06 | 2.53 ± 0.03 | 129.08 |
| HgCl2 (1 mM) | 3.49 ± 0.08 | 88.35 | 2.37 ± 0.02 | 120.92 |
| FeCl2 (1 mM) | 11.31 ± 1.02 | 286.33 | 6.88 ± 0.76 | 351.02 |
| FeCl3 (1 mM) | 4.89 ± 0.10 | 123.8 | 2.46 ± 0.02 | 125.51 |
| BaCl2 (1 mM) | 3.88 ± 0.08 | 98.23 | 2.21 ± 0.01 | 112.76 |
| PbCl2 (1 mM) | 4.2 ± 0.11 | 106.33 | 2.27 ± 0.02 | 115.82 |
| LiCl (1 mM) | 4.01 ± 0.10 | 101.52 | 2.31 ± 0.03 | 117.86 |
| NiCl2 (1 mM) | 4.12 ± 0.09 | 104.3 | 1.95 ± 0.03 | 99.49 |
| MnCl2 (1 mM) | 4.1 ± 0.11 | 103.8 | 2.54 ± 0.02 | 129.59 |
| EDTA (10 mM) | 2.59 ± 0.12 | 65.57 | 2.13 ± 0.04 | 108.67 |