Fig 1.

The post-thaw viability (A) and plating efficiency (B) of hASCs cryopreserved using undefined and defined cryopreservation media. The conventional undefined cryopreservation medium consisted of 10% DMSO+10% DMEM/F12+80% FBS while defined 1 freezing medium was composed of 3.5% DMSO+3.5% EG in DMEM/F-12 containing 0.25 M trehalose, 2% PVA, 5% ficoll, and 0.1 mM EGTA. Defined 2 freezing medium was similar to defined 1 medium but additionally contained two antioxidants (i.e., 3 mM reduced glutathione+5 mM ascorbic acid 2-phosphate). Data shown are mean±SEM. Columns with different letters in each thawing category are significantly different (p < 0.05).