Fig 2.

The post-thaw viability (A) and plating efficiency (B) of hASCs after further optimization of the defined cryopreservation medium. The undefined cryopreservation medium consisted of 10% DMSO+10% DMEM/F12+80% FBS while defined 2 medium was made up of 3.5% DMSO+3.5% EG+3 mM reduced glutathione+5 mM ascorbic acid 2-phosphate in DMEM/F-12 containing 0.25 M trehalose, 2% PVA, 5% ficoll, and 0.1 mM EGTA. Defined 3 cryopreservation medium had a slightly increased concentration (5%) of DMSO and EG; otherwise it was similar to defined 2 medium). Data shown are mean±SEM. Columns with different letters in each thawing category are significantly different (p < 0.05).