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. 2016 Mar 24;11(3):e0152017. doi: 10.1371/journal.pone.0152017

Fig 4. Effect of AQHAR on key regulators of cell cycle arrest and apoptosis in U2OS and TIG-3 cells.

Fig 4

Cells were treated with AQHAR for 24–48 h and subjected to (A) Western blotting and (B) immunostaining for cell cycle regulatory proteins (p53, p21, Cyclin B1 and p-Rb). (C) Western blot analysis revealed activation of apoptotic signaling in U2OS cells. Quantitation of the results from three independent experiments is shown as mean ± SD with statistical significance as *p<0.05 between the control and AQHAR-treated cells.