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. 2016 Mar 24;11(3):e0152012. doi: 10.1371/journal.pone.0152012

Fig 2. Shizukaol D inhibited the growth of liver cancer cells.

Fig 2

(A-D). Cells were seed in a 96 well plate and treated with a series concentration of shizukaol D. Cell proliferation was measured by CCK-8 assay. (A) The viability of Focusand SMMC-7721cells were decreased when the cells were treated with increasing concentration of shizukaol D for 48h.(B) The viability decreased faster when treated with shizukaol D than treated with same concentration of 5-FU in SMMC-7721 cells for 48 h. The cell viability decreased in Focus (C) and SMMC-7721 (D) cells treated by shizukaol D in a dose- and time-dependent manner. (E) The transparent version of Focus cells treated with 0.00, 12.50, 25.00 and 50.00 μmol/L of shizukaol D for 48 hours. (F) Colony formation assay of SMMC-7721 cells. The cells were treated with 0.00, 3.13, 6.25, 12.50 and 25.00 μmol/L of shizukaol D for 48 hours and cells for each concentration were collected respectively, and plated in 6-well plates as 500/well. The cells were cultured with normal medium for 10 to 15 d then. (G) Statistics of cloning efficiency in Figure 2F. **: P<0.01. Values are presented as mean±S.D.