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. 2016 Mar 24;11(3):e0152012. doi: 10.1371/journal.pone.0152012

Fig 3. Shizukaol D inducedliver cancer cells to apoptosis.

Fig 3

(A) The statistics of the sub-G1 ratio of Focus by FCM. Cells were incubated with shizukaol D for 48 hours before being collected and diluted in PBS with 50μg/ml PI and 0.03% TritonX-100. **: P<0.01. Values are expressed as mean±S.D. (B) Western blot analysis of the expression of PARP. Focus cells were treated with shizukaol D for 48 hbefore collected and then the total protein was subjected to 10% SDS-PAGE and transferred onto the nitrocellulose membrane. β-actin antibody was used to confirm equivalent loading.