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. 2016 Mar 24;11(3):e0152012. doi: 10.1371/journal.pone.0152012

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© 2016 Tang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — Actviation of Wnt pathway rescued inhibitory effect of shizukaol D on liver cancer cells. (A) The cell viability of SMMC-7721 cells treated by shizukaol D increased when incubated with wnt3a conditioned medium. (B) The cell viability of SMMC-7721 cells treated by shizukaol D increased when transfected with wt-β-catenin or mut-β-catenin. The expression of β-catenin protein of corresponding sample was analyzed by western blots and illustrated below. β-actin monoclonal antibody was used to confirm equivalent loading.