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. 2016 Mar 24;11(3):e0152013. doi: 10.1371/journal.pone.0152013

Fig 4. Mutation of potential operator site in umuDAb-ddrR promoter dysregulates UmuDAb-regulated gene expression.

Fig 4

(A) Underlined nucleotides are those previously identified as possible regulatory protein binding sites (an SOS box) in ADP1, due to their palindromic nature [11]. The numbering system represents the number of nucleotides upstream of the umuDAb coding region. Nucleotides -66 through -45 (similar but non-identical in A. baumannii) were identified as required for UmuDAb binding to A. baumannii DNA fragments in vitro [10]. Mutations in ADP1 mutant strains JH100-104 are represented in red boxes. RT-qPCR experiments measured ddrR (B) and umuDAb (C) expression in uninduced or induced (2 μg/mL MMC) wild type ADP1 vs mutant cells. Each gene was assayed in one RT-qPCR experiment (plate), with error bars indicating standard error of the mean from technical triplicates of biological triplicates. Statistical significance in a Student’s t-test is indicated by the symbol * for p values < 0.05, and by the symbol ** for p values < 0.01.