| CL-N |
Amplification from pTW1 to construct mutant strains |
ATAGTGTTGGTATGATGCG |
| ToJH1Hd |
Amplification from pTW1 to construct mutant strains |
GACAAGCTTAGAGTTGAATA |
| TJ2b |
JHTW1 (umuDAbΔ2–59) construction |
GTCCAGGCGATACAGCCCAA |
| TJ1 |
JHKW1 (umuDAbΔ2–83) construction |
CATCAGCCCTCCTAACACAA |
| umuD’For |
JHKW1 (umuDAbΔ2–83) construction |
GGTTTGCCATCACCTGCACA |
| CL0 |
JHDS1 (umuDAb A83Y) construction |
AGCCAACTAAAGTCATTCG |
| CL10 |
JHDS1 (umuDAb A83Y) construction |
TTTTCATCCGCCTAAAG |
| CLK |
JHDS1 (umuDAb A83Y) construction |
TAACGCATAGGTTTCAGATTG |
| a118c-a119c_g122c_For |
JHTW2 (umuDAb K40P R41P) construction |
CGAGTGCCAGAATCTCAGGTTGCTTTTATTCCGCCTTGGCTTTTAGATAAC |
| a118c-a119c_g122c_Rev |
JHTW2 (umuDAb K40P R41P) construction |
GTTATCTAAAAGCCAAGGCGGAATAAAAGCAACCTGAGATTCTGGCACTCG |
| DSumuDfor |
JHDT1 (umuDAb K40P R41P A83Y) construction |
TCTTATTGATTTTAATTCGGC |
| CL-4 |
JHDT1 (umuDAb K40P R41P A83Y) construction |
CCTGCTTATGCAATGACAG |
| ExtraUDRev |
JHDT1 (umuDAb K40P R41P A83Y) construction |
GCCTGGACTTTCAGTGC |
| To81Rev |
JHDT1 (umuDAb K40P R41P A83Y) construction |
CTGAACGTATTTGATTGAGC |
| g70a |
JHMP1 (umuDAb E24K) construction |
CTGGACGTAAGGCCAAATACCAAAAGCCAACTAAAGT |
| g70 |
JHMP1 (umuDAb E24K) construction |
ACTTTAGTTGGCTTTTGGTATTTGGCCTTACGTCCAG |
| SDMFirF |
JH100 promoter mutation construction |
TTATCGTGCGTCTCTCAACGTTTGTAACGA |
| SDMFirR |
JH100 promoter mutation construction |
TCGTTACAAACGTTGAGAGACGCACGATAA |
| SDMPromIR2For |
JH101 promoter mutation construction |
TGAATTTGTAACGATGAGCTAGCAGATTATTTTAACTTG |
| SDMPromIR2Rev |
JH101 promoter mutation construction |
CAAGTTAAAATAATCTGCTAGCTCATCGTTACAAATTCA |
| SDMFir1AFor |
JH102 promoter mutation construction |
ATTATCGTGCGTCTCTTTGAATTTGTAACGA |
| SDMFir1ARev |
JH102 promoter mutation construction |
TCGTTACAAATTCAAAGAGACGCACGATAAT |
| SDMPstPromFor |
JH103 promoter mutation construction |
CTTGAATCTGCAGCGATTTCAAGTTAGATT |
| SDMPstPromRev |
JH103 promoter mutation construction |
GAAATCGCTGCAGATTCAAGTTGACGCACGATAA |
| SDMMidEndFor |
JH104 promoter mutation construction |
AATAATCTAACTTGGCGCACTTACAAATCAAGT |
| SDMMidEndRev |
JH104 promoter mutation construction |
ACTTGAATTTGTAAGTGCGCCAAGTTAGATTATT |
| 2731RTFor2 |
JH100-JH104 promoter mutation construction |
ACGATGGGCATGGATGAAGTGG |
| 17TJ1analog |
17978 umuDAbˊ construction |
CATAATCGCCTCCATTTCAC |
| 17TJ1analogFor |
17978 umuDAbˊ construction |
GGTTTCCCATCACCAGC |
| E.coliNHisumuDS |
E. coli umuD expression |
ACCCACGCGCATGTCGTAAAAAGCACCCAATTGTTTATCAAGCCTGC |
| E.coliNHisumuDAS |
E. coli umuD expression |
CTTGGTTAGTTAGTTATTATCAGCGCATCGCCTTAACGA |
| ddrR#RTFor |
RT-qPCR on ddrR
|
ATACCGAACAAGCCGAGCAT |
| ddrR#2RTRev |
RT-qPCR on ddrR
|
AGGCATGACTAAAGCCAGCA |
| umuDAb#RTFor |
RT-qPCR on umuDAb
|
GGAGCATGTCGAGCAGAGTC |
| umuDAb#2RTRev |
RT-qPCR on umuDAb; also inJH100-JH104 promoter mutation construction |
TCACCTGCTTTGGCCGTAAT |
| 0445RTFor |
RT-qPCR on gst (ACIAD0445) |
ACCTGTACTCACTGATGGCG |
| 0445RTRev |
RT-qPCR on gst (ACIAD0445) |
ACAGACCTCGTTTCGGATCA |
| ADP0724RTFor |
RT-qPCR on nrdA
|
ATGACCGTCGTCGTACTCAC |
| ADP0724RTRev |
RT-qPCR on nrdA
|
GCTGTGCAAATTCTTCGCCA |
| 16SrRNA#RTFor |
RT-qPCR reference primer |
CCACACTGGGACTGAGACAC |
| 16SrRNA#2RTRev |
RT-qPCR reference primer |
AACCAGGTAAGCCTCCTCCT |
