Table 1. Nucleotide sequence of PCR primers and PCR conditions.
Genes | Primer sequence (5'-3') | PCR conditions | Length (bp) |
---|---|---|---|
NMB | Forward CATGACCCTGCGGGCTAGG | 94°C 5min, (94°C 30s, 58°C 30s, 72°C 30 s) 40 cycles, 72°C 7min | 567 |
Revers GAAGCAAGACATACAGCAGGGACG | |||
NMBR | Forward ATGCCCCCCAAGTCTCTTTC | 94°C 5min, (94°C 30s, 55°C 30s, 72°C 30 s) 40 cycles, 72°C 7 min | 1173 |
Revers TCACAGTGCCATTTCCTGTTTC |