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. 2016 Mar 24;11(3):e0151871. doi: 10.1371/journal.pone.0151871

Table 1. Nucleotide sequence of PCR primers and PCR conditions.

Genes Primer sequence (5'-3') PCR conditions Length (bp)
NMB Forward CATGACCCTGCGGGCTAGG 94°C 5min, (94°C 30s, 58°C 30s, 72°C 30 s) 40 cycles, 72°C 7min 567
Revers GAAGCAAGACATACAGCAGGGACG
NMBR Forward ATGCCCCCCAAGTCTCTTTC 94°C 5min, (94°C 30s, 55°C 30s, 72°C 30 s) 40 cycles, 72°C 7 min 1173
Revers TCACAGTGCCATTTCCTGTTTC