Table 1.
Purification table for CAT.
| Fractions | Activity (×105 U mL−1) |
Protein (mg mL−1) |
Specific activity (×105 U mg−1) |
Fold purification |
Yield % |
|---|---|---|---|---|---|
| crude extract | 1.2 ± 0.5 | 2.9 ± 0.8 | 0.41 ± 0.10 | 1.0 | 100 |
| sup-1a | 1.7 ± 0.3 | 1.8 ± 0.5 | 0.94 ± 0.20 | 2.3 | 88.6 |
| pellet-1a | 2.7 ± 0.8 | 5.4 ± 1.0 | 0.50 ± 0.09 | 1.2 | 10.8 |
| sup-2b | 2.3 ± 0.4 | 1.4 ± 0.3 | 1.64 ± 0.60 | 4.0 | 75.0 |
| pellet-2b | 0.3 ± 0.1 | 7.2 ± 1.2 | 0.04 ± 0.01 | 0.1 | 12.4 |
| 50% pelletc | 2.4 ± 0.7 | 7.2 ± 1.9 | 0.33 ± 0.10 | 0.8 | 10.1 |
| 80% pelletc | 3.3 ± 1.2 | 1.4 ± 0.5 | 2.36 ± 0.50 | 5.8 | 62.5 |
| HIC purifiedd | 14.7 ± 1.4 | 0.5 ± 0.1 | 29.40 ± 1.42 | 71.7 | 54.4 |
| SEC purified | 39.6 ± 2.0 | 0.6 ± 0.1 | 66.00 ± 3.97 | 160.9 | 47.0 |
asup-1 and pellet-1 are supernatant and pellet of 10,000 ×g centrifugation.
bsup-2 and pellet-2 are supernatant and pellet of 20,000 ×g centrifugation.
c50% and 80% pellet are (NH4)2SO4 precipitated fractions.
dHIC purified fraction was eluted at 250 mM (NH4)2SO4 concentration.
Each value represents the mean ± standard error at p < 0.05.