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. 2016 Mar 24;11(3):e0152273. doi: 10.1371/journal.pone.0152273

Fig 4. Construction and verification of deletion mutants Foc-ΔSIX1.

Fig 4

A: Foc-SIX1 gene deletion and replacement with an intact selectable marker gene (hph) by homologous recombination. B: Confirmation of the correct deletion by PCR. Numbers 1, 2, 3 and 4 represent the four primer pairs that specify the SIX1 gene, hph gene, and the correct upstream and downstream homologous recombination, respectively. C: Southern hybridization analysis of wild type isolate and mutants Foc-ΔSIX1. The genomic DNAs from the wild type Foc isolate and deletion mutants Foc-ΔSIX1 were digested with Sal I. A fragment amplified from upstream of the target gene was used as probe.