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. 2016 Mar 24;11(3):e0152273. doi: 10.1371/journal.pone.0152273

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© 2016 Li et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — A: Foc-ΔSIX1 mutants were complemented by transformation with approximately 3.0 kb of Foc-SIX1 spanning from -1838 bp (promoter relative to start codon) to 1132 bp (ORF and 3’ UTR). B: The SIX1 gene, hph gene and Neo gene fragments were amplified by PCR to confirm the correct complementation mutants. C: Disease symptoms of cabbage seedlings inoculated with wild type isolate Foc, deletion mutants Foc-ΔSIX1, and complementation mutants Foc-ΔSIX1::Foc-SIX1 were shown at 14 dpi. Similar results were obtained in three independent biological replicates.