Table 1. Purification of maltase from endosperm extracts.
Protein | Specific activity | Total activity | Recovery | Fold purification | |
---|---|---|---|---|---|
(mg) | (nmol μg-1 min-1) | (μmol min-1) | (%) | ||
Crude | 45 | 0.1 | 3.6 | 100 | 1 |
20–80% ammonium sulfate | 30 | 0.1 | 4 | 111 | 1.7 |
MonoQ (unbound) | 6 | 0.4 | 2 | 56 | 4.5 |
MonoS, peak Id | 0.025 | 13.5 | 0.34 | 9.5 | 168 |
Recovery is the percentage of the initial (crude extract) activity remaining at each step of the purification. Fold purification is the fold increase in specific activity relative to the crude extract at each stage of the purification.