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. 2016 Mar 24;11(3):e0151642. doi: 10.1371/journal.pone.0151642

Table 1. Purification of maltase from endosperm extracts.

Protein Specific activity Total activity Recovery Fold purification
(mg) (nmol μg-1 min-1) (μmol min-1) (%)
Crude 45 0.1 3.6 100 1
20–80% ammonium sulfate 30 0.1 4 111 1.7
MonoQ (unbound) 6 0.4 2 56 4.5
MonoS, peak Id 0.025 13.5 0.34 9.5 168

Recovery is the percentage of the initial (crude extract) activity remaining at each step of the purification. Fold purification is the fold increase in specific activity relative to the crude extract at each stage of the purification.