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. 2016 Mar 24;11(3):e0152278. doi: 10.1371/journal.pone.0152278

Fig 7. Involvement of ANKLE1 in Holliday junction resolution.

Fig 7

(A) mRNA expression profile of Ankle1 and known proteins involved in Holliday junction resolution after double strand break repair in somatic cells, representative end point PCR, Actin and Gapdh serve as loading control; (B) Western blot and qPCR analysis of iMEFs 48 h after transfection with indicated siRNAs; Scatter plot of sister chromatid exchange (SCE) frequencies in (C) Wild-type iMEFs and (D) Wild-type vs. Ankle1Δ/Δ iMEFs following siRNA treatment, each dot represents one metaphase spread, horizontal bars represent the mean, error bars: standard error of the mean, p values were determined using the students t test, n.s. not significant, * significant (p≤0.05), ** very significant (p≤0.01), *** extremely significant (p≤0.001).