Skip to main content
. 2016 Mar 24;14(3):e1002412. doi: 10.1371/journal.pbio.1002412

Fig 2. DNA double strand break repair is delayed in rmh-1 mutant.

Fig 2

(B–C) Immunostaining for DNA strand exchange protein RAD-51, with insets for mid pachytene (MP) and late pachytene (LP) nuclei. (B) Almost no RAD-51 foci are detected in LP nuclei in wild-type (WT). (C) Abundant RAD-51 foci are still detected in LP nuclei in the rmh-1(jf54) mutant, but disappear in diplotene. (B′,C′) Quantification of the numbers of RAD-51 foci per nucleus. For quantification, gonads were divided into six equal zones (A). In both WT and rmh-1(jf54), zones 1 and 2 correspond to the mitotic zone; zone 3 to transition zone and early pachytene; zones 4 to 5 to pachytene. Analysis of n = 124–231 nuclei per zone for each genotype. Distribution of RAD-51 foci is significantly different between WT and mutant for the zones 3 to 6 (Mann Whitney test, **** p < 0.0001). Scale bar = 20 μm for gonads and 5 μm for insets.