Figure 7.

Cell proliferation, cell migration, wound healing, and cell invasion of spheroids cells compared to the parental cells. (A) Spheroids showed a higher cell proliferation rate as compared to their parental cells. Cell proliferation assay was carried out from day 3 to day 24 using alamarBlue assay. (B) Spheroids had a higher migration rate than their parental cells. Cells were seeded at 10,000 cells per insert of a 24-well plate and allowed to migrate toward serum-present medium for 24 hours. Migratory cells on the bottom of the PET membrane were then stained (right) and later dissolved and the percentage of migrated cells were quantified in a microplate reader (left). (C) Spheroids recovered the “gap” created during the scratch assay more efficiently than parental cells. The percentage of wound closure of parental and spheroids (left). Microscopic images of the wound closure at 0h, 6h, 12h, and 24h post-wound initiation (right). (D) Spheroids invaded more into the matrigel than parental cells as shown by microscopy. Quantification of cell invasion showed the mean ± standard deviations of three independent experiments. Invading cells were fixed in formaldehyde and stained with crystal violet. All data were expressed as mean ± standard deviations and *p< 0.05 compared with parental cells. Magnification at 10X.