Figure 1.

Evaluation of oxidative stress markers in serum from mothers [A] and umbilical cord blood [B]. A1 and B1: Total reduced thiols determined by the DTNB method. A2 and B2: 8-iso-PGF2α evaluated by EIA method. A3 and B3: H₂O₂ scavenging capacity after incubation with 20 μM hydrogen peroxide for 30 minutes at 37°C. Hydrogen peroxide not degraded by the samples was determined by Amplex Red/horseradish peroxidase fluorescence assay. Values as mean ± SEM, *p < 0.05 vs others groups; ** p < 0.05 vs appointed study group. n = 15/group.