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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Structure. 2015 Nov 19;23(12):2267–2279. doi: 10.1016/j.str.2015.10.010

Figure 2. Evaluation of c-Myc1–88-Pin1 Affinities by Surface Plasmon Resonance.

Figure 2

Sensorgrams and 1:1 Langmuir fits from steady-state evaluation show binding of Pin1 (red), Pin1WW (green), and Pin1PPIase (blue) to both unphosphorylated (A–C) and phosphorylated c-Myc1–88 (D–F). For all measurements c-Myc1–88 was immobilized and Pin1, Pin1WW, or Pin1PPIase were injected over the surface. SPR experiments are further detailed in Figure S2.