FIGURE 11.

INT-777 increases pancreatic β cell proliferation in db/db mice. A, immunohistochemistry of pancreatic tissue sections using antibodies against Ki67 (green) and insulin (red). Representative image of n = 5; arrows indicate Ki67-positive cells. Scale bars, 100 μm. B, effect of INT-777 on β cell proliferation, expressed as % of β cells analyzed. C, INT-777 increases insulin staining intensity of β cells in db/db mice. Representative immunohistochemistry images of pancreatic tissue sections using antibody against insulin (green) in db/db mice treated with vehicle (DMSO) or INT-777 (30 mg/kg/d) intraperitoneally for 7 weeks and quantitation of the intensity of insulin staining. Data are expressed as mean fluorescence intensity (n = 6). *, p < 0.05, compared with db/db mice. Scale bars, 100 μm.