FIGURE 2.

Kinetic experiments of coupled folding and binding reactions under pseudo-first order conditions. A, example kinetic traces for two-state and three-state processes, fit to single exponential and double exponential decay functions, respectively. a. u., arbitrary units. B, dependence of observed rate constants upon protein concentrations. Analytical solutions are presented for a two-state reaction (k_on = 40 μm⁻¹ s⁻¹, k_off = 10 s⁻¹) and a three-state IF reaction (k₊ = 40 μm⁻¹ s⁻¹, k₋ = 10 s⁻¹, k_f = 10 s⁻¹, k_u = 20 s⁻¹). C, reaction schemes for two-state and three-state (IF and CS) processes.