Transmission electron microscopy and immunofluorescence microscopy of C. kronotskyensis.
A, transmission electron microscopy of C. kronotskyensis grown on dilute acid-pretreated P. trichocarpa × P. deltoides showing the cytoplasmic membrane (cm), peptidoglycan (pg), and S-layer (s). B and C, confocal microscopy of C. kronotskyensis grown on laminarin labeled with total IgY antibodies raised against Calkro_0111 TM7. C and D, confocal microscopy of C. kronotskyensis grown on xylooligosaccharides labeled with total IgY antibodies raised against Cakro_0402 TM1. The cell counterstain (DAPI) is shown in magenta, and secondary antibody labeling (DyLight488 goat anti-chicken) is shown in green. Scale bars, 100 nm (A), 4 μm (B), 1 μm (C), 4 μm (D), and 1 μm (E).