FIGURE 7.

Increased xylan solubilization and xylan attachment by Calkro_0402 knock-in C. bescii strain RKCB103. A, total biomass solubilization of washed oat spelt xylan, washed birchwood xylan, and unwashed birchwood xylan after 45.5 h of growth of C. bescii strain JWCB018 or RKCB103. B, xylose measured in the acid-hydrolyzed culture supernatant harvested at 45.5 h. Error bars, S.D. (n = 3). C. bescii strain JWCB018 cultures on washed birchwood xylan appear very turbid (C), and C. bescii strain RKCB103 cultures appear much less turbid (D) while having the same cell density/ml of culture. C. bescii strain RKCB103 appears to be more readily attached to the insoluble washed birchwood xylan substrate, suggesting that the expression of Calkro_0402 in this strain is playing a role in tethering the cells to the xylan substrate.