FIGURE 10.

SFN strongly inhibits viability and resorption of osteoclasts. l-SFN and dl-SFN effects on RAW 264.7 cells on proliferation/viability after 24 h of treatment are shown in A. For RAW 264.7 cells, the EC₅₀ for l-SFN is ∼10.10 μm and for dl-SFN is ∼6.01 μm (B). SFN affects cell metabolic activity most strongly in RAW 264.7 cells when compared with cells of the osteoblastic lineage (C). The effect of 3 μm l- or dl-SFN on dentin resorption by primary mouse osteoclast cultures is shown in D. A representative image of osteoclast resorption trails and pits on dentin is shown in E. Cell proliferation in A is measured by cell count. EC₅₀ in B is measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-like assay. For A and B, n = 4, and for C, n = 9. Bars represent mean ± S.D.; error bars represent S.D. **, p ≤ 0.01; ***, p ≤ 0.001.