FIGURE 5.

Removal of both putative sorting motifs enhances cell surface display of full-length E3/49K and C-terminal fragments and reduces their accumulation in swollen vesicles during chloroquine treatment. A549 cells were transiently transfected with pSG5 plasmids encoding WT and mutant E3/49K proteins as described under “Experimental Procedures.” Cells were treated with 100 μm chloroquine for 12 h (B) or left untreated (A). After 36 h, cells were processed for confocal laser microscopy. Staining was performed with rat mAb 4D1 directed against the N-terminal ectodomain (αN) and rabbit serum R25050 against the C terminus of E3/49K (αC-term), followed by donkey anti-rabbit IgG FITC and donkey anti-mouse IgG Rhodamine Red-X. The figure shows a representative experiment of three.