Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 28;291(13):6813–6830. doi: 10.1074/jbc.M115.678953

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 9. — Enzyme kinetic analysis of activated RipAY and Gcg1. Shown is a Michaelis-Menten plot of Trx-h5-activated RipAY (A) and Gcg1 (B) toward glutathione. Gcg1 (0.8 μm) or RipAY (0.04 μm) in the presence of Trx-h5 (8 μm) was used for determination of kinetic parameters. Different concentrations of glutathione were used ranging from 0.5 to 20 mm. Dug1-coupled assay was used for the study as described under “Experimental Procedures.” Data of three independent experiments were analyzed by non-linear regression using GraphPad prism version 6.0. Error bars, S.E.