TABLE 2.
S. cerevisiae plasmids used in this study
| Plasmids | Construct | Reference or source |
|---|---|---|
| pMT751 | URA3, 2μ, PGAL1-attR1-Cmr-ccdB-attR2-GFP | Ref. 33 |
| pMT830 | URA3, 2μ, Ptet-off-attR1-Cmr-ccdB-attR2-GFP | Ref. 33 |
| pMT1373 | URA3, 2μ, PGAL1-attR1-Cmr-ccdB-attR2-Protein A | This study |
| pGBKT7 | TRP1, 2μ, yeast two-hybrid GAL4 DNA-BD vector | Clontech |
| pGADT7 | LEU2, 2μ, yeast two-hybrid GAL4 AD vector | Clontech |
| pACT2 | LEU2, 2μ, yeast two-hybrid GAL4 AD vector | Clontech |
| pMT731 | pACT2; GAL4 AD-attR1-Cmr-ccdB-attR2 | This study |
| pMT922 | pMT830; URA3, 2μ, Ptet-off-GFP | Ref. 33 |
| pRE85 | pMT751; URA3, 2μ, PGAL1-R. sonalacearum RipAY-GFP | This study |
| pRE97 | pMT751; URA3, 2μ, PGAL1-R. sonalacearum RSc0782-GFP | This study |
| pRE98 | pMT751; URA3, 2μ, PGAL1-H. sapiens Chac2-GFP | This study |
| pRE99 | pMT751; URA3, 2μ, PGAL1-P. syringae PSPTO_5239-GFP | This study |
| pRE100 | pMT751; URA3, 2μ, PGAL1-S. cerevisiae GCG1-GFP | This study |
| pRE161 | pMT751; URA3, 2μ, PGAL1-A. citrulli Aave_4606-GFP | This study |
| pRE173 | pMT751; URA3, 2μ, PGAL1-A. thaliana AT1G44790/GGCT2;3-GFP | This study |
| pRE174 | pMT751; URA3, 2μ, PGAL1-A. thaliana AT4G31290/GGCT2;2-GFP | This study |
| pRE175 | pMT751; URA3, 2μ, PGAL1-A. thaliana AT5G26220/GGCT2;1-GFP | This study |
| pRE86 | pMT751; URA3, 2μ, PGAL1-RipAYE216Q-GFP | This study |
| pRE164 | pMT751; URA3, 2μ, PGAL1-RipAY2Y129A-GFP | This study |
| pRE165 | pMT751; URA3, 2μ, PGAL1-RipAYL130A-GFP | This study |
| pRE166 | pMT751; URA3, 2μ, PGAL1-RipAYS131A-GFP | This study |
| pRE167 | pMT751; URA3, 2μ, PGAL1-RipAYL132A-GFP | This study |
| pRE168 | pMT751; URA3, 2μ, PGAL1-RipAYL130G-GFP | This study |
| pSF38 | pMT751; URA3, 2μ, PGAL1-RipAYC333S-GFP | This study |
| pRE257 | pMT1373; URA3, 2μ, PGAL1-S. cerevisiae GCG1-Protein A | This study |
| pRE258 | pMT1373; URA3, 2μ, PGAL1-RipAY-Protein A | This study |
| pRE259 | pMT1373; URA3, 2μ, PGAL1-RipAYE216Q-Protein A | This study |
| pSF25 | pGBKT7; TRP1, 2μ, PADH1-GAL4 DNA-BD-RipAYE216Q | This study |
| pSF37 | pGBKT7; TRP1, 2μ, PADH1-GAL4 DNA-BD-RipAYE216Q,C333S | This study |
| pRE249 | pGADT7; LEU2, 2μ, PADH1-GAL4 AD-A. thaliana TRX h2 | This study |
| pRE250 | pGADT7; LEU2, 2μ, PADH1-GAL4 AD-A. thaliana TRX h3 | This study |
| pSF50 | pMT731; LEU2, 2μ, PADH1-GAL4 AD-A. thaliana TRX h5 | This study |
| pSF51 | pMT731; LEU2, 2μ, PADH1-GAL4 AD-A. thaliana TRX h5C39S,C42S | This study |
| pSF52 | pMT731; LEU2, 2μ, PADH1-GAL4 AD-S. cerevisiae TRX1 | This study |
| pMT1416 | pMT731; LEU2, 2μ, PADH1-GAL4 AD-R. solanacearum TrxA | This study |
| pTEF1-416-HGT1 | pRS416; URA3, CEN, PTEF1-S. cerevisiae HGT1 | Ref. 31 |