TABLE 3.
E. coli plasmids used in this study
| Plasmids | Construct | Reference or source |
|---|---|---|
| pET23d | Amp, PT7, the C-terminal His6 tag fusion vector | Novagen, Inc. |
| pDEST17 | Amp, PT7, the Gateway N-terminal His6 tag fusion vector | Life Technologies, Inc. |
| pMT1371 | pET23d: PT7-attR1-Cmr-ccdB-attR2-Protein A | This study |
| pSF16 | pET23d: PT7-R. solanacearum RipAY-His6 | This study |
| pSF17 | pET23d: PT7-S. cerevisiae GCG1-His6 | This study |
| pSF16 | pET23d: PT7-R. solanacearum RipAY-His6 | This study |
| pSF40 | pET23d: PT7-S. cerevisiae TRX1-His6 | This study |
| pSF49 | pDEST17: PT7-Met-His6-A. thaliana TRX h5 | This study |
| pMT1293 | pET23d: PT7-S. cerevisiae DUG1-His6 | This study |
| pMT1409 | pDEST17: PT7-Met-His6-A. thaliana TRX h2 | This study |
| pMT1410 | pDEST17: PT7-Met-His6-A. thaliana TRX h3 | This study |
| pMT1411 | pDEST17: PT7-Met-His6-A. thaliana TRX h5C39S,C42S | This study |
| pMT1415 | pDEST17: PT7-Met-His6-R. solanacearum TrxA | This study |
| pMT1368 | pMT1371: PT7-Protein A | This study |
| pRE254 | pMT1371: PT7-S. crevisiae GCG1-Protein A | This study |
| pRE255 | pMT1371: PT7-R. solanacearum RipAY-Protein A | This study |
| pRE256 | pMT1371: PT7-R. solanacearum RipAYE216Q-Protein A | This study |
| pRE496 | pMT1371: PT7-R. solanacearum RipAYC333S-Protein A | This study |