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Kinetic parameters of S. cerevisiae Gcg1 and AtTrx-h5-activated RipAY
| Enzyme | Km | kcat | kcat/Km | Comparison of kcat/Km value versus RipAY |
||
|---|---|---|---|---|---|---|
| Gcg1 | AtGGCT2;2a | AtGGCT2;3a | ||||
| mm | s−1 | s−1 mm−1 | -fold | |||
| Gcg1 | 1.23 ± 0.17 | 0.33 ± 0.02 | 0.27 ± 0.03 | |||
| RipAYb | 2.11 ± 0.17 | 52.8 ± 9.58 | 25.2 ± 1.22 | 94.0 | 66.7 | 1,081 |
a Data from Kumar et al. (29).
b RipAY was activated by 8 μm AtTrx-h5.
