TABLE 1.

Primers used in the amplification of FbiB (Rv3262) constructs

Construct	Primer Sequences (5′-3′)
Full-length FbiB
Forward	GGCAGCGGCGCGTTGACCGGCCCCGAACATGGC
Reverse	GAAAGCTGGGTGTCACTTCAGGATCAGCAAATC
FbiB N-terminal domain (FbiB(11–249))
Forward	GGCAGCGGCGCGATGACCATCGAGATCCTG
Reverse	GAAAGCTGGGTGTTAGAGCGCTTCGGCGGT
FbiB C-terminal domain (FbiB(249–448))
Forward	GGCAGCGGCGCGATGACCGCCGAAGCGCTC
Reverse	GAAAGCTGGGTGTTATCACTTCAGGATCAGCAA