TABLE 4.
Small angle x-ray scattering parameters and statistics
| Data collection parameters | |
| Beamlinea | AS SAXS/WAX |
| Wavelength (Å) | 1.0322 |
| Detector | Dectris-Pilatus 1M |
| Camera length (mm) | 1600 |
| q range (Å−1) | 0.006 – 0.6 |
| Sample capillary flow rate (ml/min) | 0.5 |
| Total sample volume (μl) | 100 |
| Exposure time/image (s) | 1 |
| No. of images/sample | 20 |
| Concentration range (mg/ml) | 0.25–5.0 |
| Temperature (K) | 283 |
| Structural parametersb | |
| I(0) (cm−1) (from P(r)) | 0.557 ± 0.002 |
| Rg (Å) (from P(r)) | 39.82 ± 0.01 |
| I(0) (cm−1) (from Guinier) | 0.561 ± 0.001 |
| Rg (Å) (from Guinier) | 39.8 ± 0.2 |
| Dmax (Å) | 130.0 |
| Porod volume estimate (Å3) | 56,501 |
| Dry volume calculated from sequence (Å3) | 57,567 |
| Software | |
| Primary data collection | scatterBrain |
| Data processing | scatterBrain |
| Rigid body modelling | SASREF |
| Computation of model intensities | CRYSOL |
a Full details of the beamline specifications are available at the Australian Synchrotron website.
b For a 5 mg/ml sample.