TABLE 3.

Microarray gene profiling of the colon of Pla2g10^−/− mice versus Pla2g10^+/+ mice in DSS-induced colitis

Total RNAs were isolated from the colons of Pla2g10^+/+ (WT) and Pla2g10^−/− (KO) mice with or without 1% DSS treatment for 1 week. Equal amounts of total RNA (pooled from three mice for each genotype) were subjected to one-color gene expression microarray analysis. Data were processed using the Feature Extraction software from Agilent and analyzed using GeneSpring software. Fold changes (KO relative to WT) on the microarray are listed.

Gene name	Accession no.	DSS(−) KO/WT	DSS(+) KO/WT
Cytokines and their receptors
Il1b	NM_008361	1.74	2.92
Il6	NM_031168	0.70	2.85
Il17a	NM_010552	0.45	2.94
Il2	NM_008366	1.07	7.12
Il7r	NM_008372	1.15	2.05
Chemokines and their receptors
Ccl4	NM_013652	1.24	4.07
Ccl7	NM_013654	0.60	2.25
Ccr6	NM_009835	1.35	2.44
Cxcl13	NM_018866	1.21	5.49
Cxcl2	NM_009140	0.93	4.60
Macrophages
Emr1	NM_010130	0.76	2.24
Nos2	NM_010927	0.76	2.72
Cd68	NM_009853	0.59	2.04
Arg1	NM_007482	1.00	3.36
Chi3l3	NM_009892	1.07	3.58
Inflammation-related
S100a9	NM_001281852	3.60	5.18
S100a8	NM_013650	3.35	5.86
Ptgs2	NM_011198	1.14	2.64
Mmp3	NM_010809	0.66	2.98
Mmp9	NM_013599	0.52	3.02
Mmp10	NM_019471	0.72	3.65
Epithelial cells
Krt78	NM_212487	0.76	0.43
Krt1	NM_008473	1.33	0.15
Defb23	NM_001037933	1.03	0.08
Defb45	NM_001037752	0.99	0.25