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PAR2 induces RelA nuclear translocation. Cells grown on coverslips in serum-free media were untreated (A, C, and E) or treated with TNFα (100 ng/ml, 1.9 nm, 30 min) (B), SLIGRL-NH2 (20 μm, 85 min) (D), or KLK5 (3.23 μm, 60 min) (F). After washing and fixing/permeabilization, cells were treated with antibody to RelA, a FITC secondary, and mounted. Nuclear translocation of RelA was assessed on an inverted AMG EVOS All-In-One digital microscope.
