FIGURE 5.

Effect of glutathionylation on ATPase activity and nucleotide binding of DnaK. Data are shown for untreated control (DnaK-C), glutathionylated (DnaK-G), and deglutathionylated (DnaK-D) samples of DnaK with or without the co-chaperone DnaJ, nucleotide exchange factor GrpE, or peptide as indicated. Details of reaction conditions are described under “Experimental Procedures.” A, effect of glutathionylation on ATPase activity of DnaK measured by malachite green assay. The data shown are the mean of three independent experiments, and the error bars represent the S.E. B, effect of glutathionylation on ATP and ADP binding to DnaK. Binding to ATP-agarose and elution by ADP were detected by SDS-PAGE. RT, run-through; W, wash; E, eluate.