TABLE 4.

IC₅₀ values for selected positive hits, determined by monitoring the effect of the compounds on the rate of oxidation of coumarin boronic acid (100 μm) as a probe for H₂O₂

Differentiated HL60 cells were stimulated with PMA (1 μm) to induce Nox2 activity in HBSS supplemented with HEPES buffer (25 mm) and dtpa (0.1 mm) and the extent of probe oxidation was monitored in 384-well plates using fluorescence plate reader.

No.	Compound	IC50 (μm)
		Mean	S.D.
1	Amiodarone·HCl	11	2
2	Amoxapine	16	4
3	Benzbromarone	13	1.5
4	Benzethonium chloride	23	0.7
5	Cetylpyridinium chloride	2.3	0.3
6	Chlorpromazine·HCl	13	0.6
7	Clotrimazole	59	25
8	Diphenyleneiodonium	0.08	0.07
9	Dyclonine	39	16
10	Econazole nitrate	22	1.1
11	Hexachlorophene	2.2	0.4
12	Mefloquine·HCl	16	0.4
13	Miconazole	15	6.5
14	Mitoxantrone	18	12
15	Phenylarsine oxide	0.2	0.04
16	Promazine·HCl	31	1.6
17	Sulconazole nitrate	19	2.6
18	Suloctidil	7.7	3.5
19	Tamoxifen citrate	18	0.5
20	Thimerosal	2.2	0.6
21	Thioridazine	9.3	2.1
22	Tioconazole	9.2	0
23	Trifluoperazine	9	2.2
24	Triflupromazine·HCl	12	2.6