FIGURE 8.

NOX4-dependent H₂O₂ production in Canx-deficient MEF. A, relative diphenylene iodonium (DPI; 10 μm, 1 h) sensitive Amplex Red/HRP signal in Canx^−/− or wt MEFs after TGF-β1 treatment (10 ng/ml, 24 h). B, representative Western blot and densitometry of NOX4 (highlighted upper NOX4 band) and CANX protein expression normalized to β-Actin and wt control in Canx^−/− or wt MEFs. C, normalized, relative mRNA level of Nox4 mRNA in Canx^−/− or wt MEFs. n ≥ 3, mean ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001 relative to the corresponding wt cells; #, p < 0.05; ##, p < 0.01; ###, p < 0.001 relative to the corresponding non-treated cells.