FIGURE 11.

C. albicans killing and production of superoxide and nitric oxide are higher in cPLA₂α^−/− RPM compared with cPLA₂α^+/+ RPM. a, the ability of cPLA₂α^+/+ (WT) and cPLA₂α^−/− (KO) RPM to bind and internalize GFP-C. albicans (recognition assay) is shown. b, to measure NADPH oxidase activity, superoxide anion production by cPLA₂α^+/+ and cPLA₂α^−/− RPM infected with C. albicans or treated with PMA (320 nm) for 90 min was determined. c, nitric oxide production by cPLA₂α^+/+ and cPLA₂α^−/− RPM was measured after treatment with and without IFNγ for 18 h, followed by infection with C. albicans for 4 h. d, for measuring C. albicans killing using the XTT viability assay, cPLA₂α^+/+ and cPLA₂α^−/− RPM were treated with and without IFNγ for 18 h and then incubated with C. albicans for 4 h. e, for measuring the effect of prostaglandins in regulating C. albicans killing, cPLA₂α^+/+ RPM were incubated with and without IFNγ for 18 h and then pretreated with indomethacin (Indo; 10 μm) for 30 min prior to incubation with C. albicans for 4 h (n = 3; *, p < 0.05 compared with C. albicans-infected cPLA₂α^+/+ RPM (b), cPLA₂α^+/+ RPM treated with IFNγ (c), cPLA₂α^+/+ RPM incubated with and without IFNγ (d); and cPLA₂α^+/+ RPM not treated with indomethacin (e)). Error bars, S.E.