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. 2016 Feb 3;291(13):7070–7086. doi: 10.1074/jbc.M116.714873

FIGURE 9.

FIGURE 9.

Phosphorylation of CREB requires p38 activation but not cPLA2α/COX-1/cAMP/PKA pathway. a, COX-1−/− RPM were treated with or without 6-Bnz-cAMP (6-Bnz; 1 mm), 8-pCPT-2-O-Me-cAMP-AM (8-pCPT; 10 μm), 8-Br-cAMP (8-Br; 2 mm), or 6-Bnz together with 8-pCPT for 30 min and then infected with C. albicans for 3 h. Relative gene expression was determined by RT-PCR (n = 3; *, p < 0.05 compared with C. albicans-infected RPM not treated with cAMP analogues). b, Western blots of phospho-CREB and β-actin in cPLA2α+/+ and cPLA2α−/− RPM or of COX-1+/+ or COX-1−/− RPM treated with or without C. albicans for 10–60 min. c, Western blots of phospho-CREB and β-actin in cPLA2α+/+ and cPLA2α−/− RPM treated with and without U0126 (U; 1 μm), SB20219 (S; 10 μm), or U0126 together with SB20219 (U/S) or in COX-1+/+ and COX-1−/− RPM treated with SCH772984 (SC; 1 μm), LY222820 (L; 1 μm), or SCH772984 together with LY222820 (S/L) for 30 min and then incubated with C. albicans for 30 min. Western blotting results are representative of three independent experiments. Error bars, S.E.