Skip to main content
. 2016 Feb 9;291(13):7128–7141. doi: 10.1074/jbc.M115.668699

FIGURE 5.

FIGURE 5.

Concentration response of NADH, ADPR, and NAM. Residual activity of SIRT1–3 and SIRT6-mediated hydrolysis of Kac (LGKac), ϵ-N-octanoyllysine (TARKoct), and ϵ-N-myristoyllysine (TARKmyr) (300 μm peptide substrate (3b, 1f, and 1i), 1000 μm NAD+) as well as SIRT5-mediated hydrolysis of ϵ-N-succinyllysine (LGKsuc) and ϵ-N-glutaryllysine (LGKglu) (100 μm peptide substrate (3p and 3q), 500 μm NAD+) at different NADH, ADPR, and NAM concentrations. Conversions are based on relative areas under the relevant peaks in the UV chromatograms.