TABLE 1.

IC₅₀values for NADH, ADPR, and NAM

Inhibition constants of SIRT1–3-, SIRT6-, and SIRT5-mediated hydrolysis of relevant substrates (300 μm peptide substrate (3b, 1f, and 1i), 1000 μm NAD⁺, or 100 μm peptide substrate (3p and 3q), 500 μm NAD⁺), calculated from concentration-response experiments shown in Fig. 5.

	NADH	ADPR	NAM
	mm	mm	μm
SIRT1
3b	1.3 ± 0.2	0.22 ± 0.03	180 ± 30
1f	3.0 ± 0.1	1.9 ± 0.4	84 ± 11
1i	1.3 ± 0.4	1.1 ± 0.3	68 ± 6
SIRT2
3b	4.8 ± 0.2	4.9 ± 3.0	41 ± 6
1f	32 ± 2	3.9 ± 0.5	44 ± 5
1i	21 ± 8	8.0 ± 3.5	35 ± 4
SIRT3
3b	3.3 ± 0.4	6.0 ± 3.7	72 ± 15
1f	68 ± 9	3.9 ± 0.6	76 ± 7
1i	8.9 ± 1.9	1.1 ± 0.1	53 ± 7
SIRT6
3b	NDa	ND	ND
1f	9.3 ± 0.5	0.074 ± 0.014	150 ± 40
1i	1.8 ± 0.3	0.089 ± 0.029	120 ± 30
SIRT5
3p	3.8 ± 0.2	>10b	28 ± 5
3q	2.4 ± 0.3	4.3 ± 2.2	99 ± 30

^a ND, not determined due to the prohibitively high concentration of enzyme needed.

^b The IC₅₀ value could not be determined from the concentration-response curve (84% residual activity at 1 mm, the highest concentration tested).