FIG 3 .

Upregulation of mitochondrial expression factors occurs during the early phase of HCMV replication. (A) Time course analysis. U373 and HFFF2 cells were either mock infected or infected with HCMV (Merlin) at an MOI of 5 for the times indicated. Lysates were processed and analyzed as described in the legend to Fig. 2. Membranes were labeled with the indicated antibodies. The upregulation of mitochondrial expression factors was first observed at 24 hpi concomitant with the expression of the HCMV UL44 early protein. m., mock infected cells. (B) Induction of mitochondrial expression factors is dependent on the expression of immediate early/early but not late HCMV genes. HFFF2 cells were either mock infected or infected with an intact or a UV-inactivated HCMV (Merlin) at an MOI of 5 for 48 h.