FIG 5 .

Recombinant UspA2 and UspA2H bind to network-forming collagens IV and VI. (A and B) Binding of recombinant ¹²⁵I-labeled UspA1, UspA2 and UspA2H to collagen IV (A) or collagen VI (B) coated in microtiter plates. The means of the results of three independent experiments in triplicates are shown. The error bars indicate standard deviations. Statistical calculations were performed using two-way ANOVA. The differences between ¹²⁵I-labeled UspA2 and UspA2H and UspA1 (28 to 156 kcpm added) in binding to collagen IV and VI were statistically significant (P value range, 0.01 to 0.001). (C) TEM images of gold-labeled UspA1, UspA2, and UspA2H binding to a collagen IV-coated grid. The solid arrows indicate gold particles bound to NC domains, and dashed arrows show N7 domains of the collagen IV molecule. (D) Binding of gold-labeled UspA1, UspA2, and UspA2H to a collagen VI-coated grid as revealed by TEM. Gold particles are indicated by arrows.