Fig. 3.
HIST1H2BE knockdown and overexpression affect growth of breast cancer cells. a Relative mRNA expression of HIST1H2BE knockdown clones (sh1, sh2, sh3) compared with non-infected cells (LTED), and cells infected with scrambled control virus (SCR). Expression was analyzed using one-way ANOVA followed by Tukey’s post-test for multiple comparison (***p < 0.001). b Growth curves for control cells and cells with HIST1H2BE knockdown. Values represent means ± SEM of 2 experiments performed in technical triplicate. Growth was analyzed using non-linear regression and an exponential growth equation (***p < 0.001). c Relative mRNA expression of HIST1H2BE overexpressing clones (H2, H3) compared with non-transfected MCF-7 cells and cells transfected with vector only (−EV1 and –EV2). Measurement was performed as described in (a). d Growth of overexpressing cells (H2) compared with control cells. Cells were kept in 5 % CSS for 3 days before treatment with 10−9 M estradiol. Measurement was performed as described in (b) (**p < 0.05)