Fig. 4.

HIST1H2BE, and other histone variants, are overexpressed in endocrine-resistant breast tumors. a Relative mRNA expression of HIST1H2BE in a panel of breast cancer cell lines, presented as fold change compared with MCF-7, measured by q-RT-PCR. ER+ and ER− cell lines shown in black and white bars, respectively, and Her2 overexpression is indicated with ^‡. b Relative mRNA expression of HIST1H2BE in endocrine-sensitive and endocrine-resistant tumors, measured by q-RT-PCR. RPS11 was used as control, and primer sequences are shown in the Electronic Supplementary Material. Boxplots represent the median, 25th, and 75th percentiles of fold change in expression, with the whiskers extending to 1.5 times the range between the 25th and 75th percentiles (p = 0.055). c Heat map of histone variants expression (n = 61) and Ki67 by nanostring analysis in tumors from cases and controls